Rabbit brain-derived neurotrophic factor (BDNF) detection kit

Use: Used to detect the concentration of rabbit brain-derived neurotrophic factor (BDNF) in samples such as serum, plasma, and cell culture supernatant.Specificity: This kit uses double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In the microwell ELISA plate pre-coated with anti-rabbit brain-derived neurotrophic factor (BDNF) antibody (solid phase antibody), add rabbit brain-derived neurotrophic factor (BDNF) calibrator and the sample to be tested, then add biotin-labeled antibody, after incubation and sufficient washing, add HRP-coupled avidin, after incubation and sufficient washing, remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which is finally converted into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on the microplate reader. The absorbance (OD value) is positively correlated with the concentration of rabbit brain-derived neurotrophic factor (BDNF) in the sample to be tested. Fitting the calibrator curve can calculate the concentration of rabbit brain-derived neurotrophic factor (BDNF) in the sample.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.