Rabbit S100 Calcium Binding Protein A12 (S100A12) Detection Kit

Use: Used to detect the concentration of rabbit S100 calcium binding protein A12 (S100A12) in samples such as serum, plasma, and cell culture supernatant.Specificity: This kit uses a double antibody sandwich enzyme-linked immunosorbent assay (ELISA). In a microwell ELISA plate pre-coated with anti-rabbit S100 calcium-binding protein A12 (S100A12) antibody (solid phase antibody), add rabbit S100 calcium-binding protein A12 (S100A12) calibrator and the sample to be tested, then add biotin-labeled antibody, incubate and fully wash, then add HRP-coupled avidin, incubate and fully wash to remove unbound components, and form a sandwich complex of solid phase antibody-antigen-biotin-labeled antibody-avidin enzyme on the solid phase surface of the microwell plate. Add TMB colorimetric solution to produce a blue product, which eventually turns into yellow under the action of the stop solution. The absorbance (OD value) is measured at a wavelength of 450nm on an ELISA instrument. The absorbance (OD value) is positively correlated with the concentration of rabbit S100 calcium-binding protein A12 (S100A12) in the sample to be tested. The concentration of rabbit S100 calcium binding protein A12 (S100A12) in the sample can be calculated by fitting the calibration curve.

Storage Conditions
Store at 2-8°C.

Use: The kit is for research use only.