Rad52 (phospho-Y104) polyclonal, anti-human, mouse, rat
€328.00
In stock
SKU
BS4869
Background:
DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved.
Alternative Name:
DNA repair protein RAD52 homolog, RAD52
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-Rad52 (Y104) polyclonal antibody detects endogenous levels of Rad52 protein when phosphorylated at Tyr104.
Immunogen:
Synthetic phosphopeptide derived from human Rad52 around the phosphorylation site of Tyrosine 104.
MW: ~ 46 kDa
Swis Prot.: P43351
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved.
Alternative Name:
DNA repair protein RAD52 homolog, RAD52
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: p-Rad52 (Y104) polyclonal antibody detects endogenous levels of Rad52 protein when phosphorylated at Tyr104.
Immunogen:
Synthetic phosphopeptide derived from human Rad52 around the phosphorylation site of Tyrosine 104.
MW: ~ 46 kDa
Swis Prot.: P43351
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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