Rank Ligand human active recombinant protein
€295.00
In stock
SKU
600166
Protein Family: Cytokines
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: tumor necrosis factor ligand superfamily member 11, receptor activator of nuclear factor kappa B ligand RANKL, TNF-related activation induced cytokine, TRANCE, osteoprotegerin ligand, OPGL, osteoclast differentiation factor, ODF, CD254, Tumor necrosis factor ligand superfamily member 11, membrane form, tumor necrosis factor ligand super family member 11, soluble form, TNFSF11, OPGL, RANKLDescription:
RANK ligand (RANKL) is a cytokine that binds to TNFRS11B/OPG and to TNFGS11A/RANK This cytokine also augments the ability of dendritic cells to stimulate naïve T-cell proliferation, is an important regulator of interactions between T-cells and dendritic cells, and plays a role in the regulation of the T-cell-dependent immune response. RANKL is an osteoclast differentiation and activation factor and enhances bone resosrption in humoral hypercalcemia of malignancy. Recombinant human soluble RANKL produced in E. coli is a single non-glycosylated polypeptide chain containing 176 amino acids with a MW of 20,006 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
RANK ligand is fully biologically active when compared to standard. The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0-10.0 ng/ml and by dose dependant stimulation of IL-8 production in human PBMC.
Accession No.: O14788
MW: 20006 Da
Sequence:
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium phosphate pH8.0. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: tumor necrosis factor ligand superfamily member 11, receptor activator of nuclear factor kappa B ligand RANKL, TNF-related activation induced cytokine, TRANCE, osteoprotegerin ligand, OPGL, osteoclast differentiation factor, ODF, CD254, Tumor necrosis factor ligand superfamily member 11, membrane form, tumor necrosis factor ligand super family member 11, soluble form, TNFSF11, OPGL, RANKLDescription:
RANK ligand (RANKL) is a cytokine that binds to TNFRS11B/OPG and to TNFGS11A/RANK This cytokine also augments the ability of dendritic cells to stimulate naïve T-cell proliferation, is an important regulator of interactions between T-cells and dendritic cells, and plays a role in the regulation of the T-cell-dependent immune response. RANKL is an osteoclast differentiation and activation factor and enhances bone resosrption in humoral hypercalcemia of malignancy. Recombinant human soluble RANKL produced in E. coli is a single non-glycosylated polypeptide chain containing 176 amino acids with a MW of 20,006 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
RANK ligand is fully biologically active when compared to standard. The activity is determined by its ability to induce osteoclast formation in RAW264.7 cells using a conc. of 5.0-10.0 ng/ml and by dose dependant stimulation of IL-8 production in human PBMC.
Accession No.: O14788
MW: 20006 Da
Sequence:
MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium phosphate pH8.0. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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