Rank Ligand mouse active recombinant protein
€295.00
In stock
SKU
600210
Protein Family: Cytokines
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: tumor necrosis factor ligand superfamily member 11, receptor activator of nuclear factor kappa B ligand RANKL, TNF-related activation induced cytokine, TRANCE, osteoprotegerin ligand, OPGL, osteoclast differentiation factor, ODF, CD254, Tumor necrosis factor ligand superfamily member 11, membrane form, tumor necrosis factor ligand super family member 11, soluble form, TNFSF11, OPGL, RANKLDescription:
RANK Ligand (RANKL) occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL, which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANKL, which is enzymatically processed by TACE. All three forms stimulate their specific receptors, TNFRSF11B/OPG and TNFRSF11A/RANK, which are located on osteoclastic and dendritic cells. RANKL is critically involved in osteoclastic function, and in the regulation of specific immunity. Recombinant M-sRANK Ligand is approximately 19.9 kDa and contains 174 amino acids.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Measured by its ability to induce osteoclast formation on mouse RAW264.7 cells using a concentration of 5.0-10.0 ng/ml.
Accession No.: O35235
MW: 19900 Da
Sequence:
PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium phosphate pH7.5, 50 mM NaCl. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: tumor necrosis factor ligand superfamily member 11, receptor activator of nuclear factor kappa B ligand RANKL, TNF-related activation induced cytokine, TRANCE, osteoprotegerin ligand, OPGL, osteoclast differentiation factor, ODF, CD254, Tumor necrosis factor ligand superfamily member 11, membrane form, tumor necrosis factor ligand super family member 11, soluble form, TNFSF11, OPGL, RANKLDescription:
RANK Ligand (RANKL) occurs in 3 forms: cell-bound RANKL, which is expressed by osteoblast lineage cells, soluble RANKL, which is expressed by activated T lymphocytes, and a truncated ectodomain form derived from the cell-bound RANKL, which is enzymatically processed by TACE. All three forms stimulate their specific receptors, TNFRSF11B/OPG and TNFRSF11A/RANK, which are located on osteoclastic and dendritic cells. RANKL is critically involved in osteoclastic function, and in the regulation of specific immunity. Recombinant M-sRANK Ligand is approximately 19.9 kDa and contains 174 amino acids.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
Measured by its ability to induce osteoclast formation on mouse RAW264.7 cells using a concentration of 5.0-10.0 ng/ml.
Accession No.: O35235
MW: 19900 Da
Sequence:
PAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID
Purity:
Purity > 95% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 10 ug protein lyophilized in 10 mM sodium phosphate pH7.5, 50 mM NaCl. Reconstitute with 0.1 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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