RANK/TNFRSF11A mouse recombinant protein

Background:
RANK, as known as Tumor necrosis factor receptor superfamily member 11A (TNFRSF11A) is a member of the tumor necrosis factor receptor (TNFR) molecular sub-family. It is the receptor for RANK-Ligand (RANKL) and part of the RANK/RANKL/OPG signaling pathway that regulates osteoclast differentiation and activation. It is associated with bone remodeling and repair, activation of NF-kappa B and c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, immune cell function, lymph node development, thermal regulation, and mammary gland development. It is able to block TRANCE induced biological activity. Recombinant mouse RANK, fused to hlgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names:
TNF receptor superfamily member 11a, Tumor necrosis factor receptor superfamily member 11A, Osteoclast differentiation factor receptor, Receptor activator of NF-KB, Familial expansile osteolysis, TRANCE receptor, RANK, CD265, FEO, ODFR, TRANCE-R, TNR11

Domain: 31-214aa
Sequence:
VTPPCTQ ERHYEHLGRC CSRCEPGKYL SSKCTPTSDS VCLPCGPDEY LDTWNEEDKC LLHKVCDAGK ALVAVDPGNH TAPRRCACTA GYHWNSDCEC CRRNTECAPG FGAQHPLQLN KDTVCTPCLL GFFSDVFSST DKCKPWTNCT LLGKLEAHQG TTESDVVCSS SMTLRRPPKE AQAYLPS

UniProt No.: O35305
NCBI Accession No.: NP_033425.3

Molecular Weight: 47.5 kDa (426aa)
Concentration: 1mg/ml (determined by absorbance at 280nm)
Formulation: Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 90% by SDS-PAGE
Bioactivity: Measured by its binding ability in a functional ELISA with Mouse RANKL/TNFSF11 (CAT# ATGP3915). The ED50 range ≤ 200 ng/ml.
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: hIgG-His-Tag
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.