Rantes human active recombinant protein
€295.00
In stock
SKU
600167
Protein Family: Cytokines
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: C-C motif chemokine 5, small inducible cytokine A5, T cell specific protein RANTES, SIS delta, T cell specific protein P228, TCP228, Eosinophil-chemotactic cytokine, EoCP, RANTES(3-68), RANTES(4-68), CCL5, D17S136E, SCYA5Description:
RANTES is a chemoattractant for blood monocytes, memory T-helper cells and eosinophils. This cytokine causes the release of histamine from basophils and activates eosinophils. RANTES binds to CCR1, CCR3, CCR4 and CCR5, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form, RANTES (3-68), acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1 infection. The second processed form, RANTES (4-168), exhibits reduced chemotactic and HIV-suppressive activity compared to RANTES(1-68 and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. Recombinant Human Rantes produced in E. coli is a single non-glycosylated polypeptide chain containing 68 amino acids with a MW of 7,809.2 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
rHuRantes is fully biologically active when compared to standard. The activity is determined by the chemoattract of human blood monocytes at a concentration between 1-8 ng/ml.
Accession No.: P13501
MW: 7809 Da
Sequence:
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 20 ug of lyophilized protein. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathway and Disease: Cell Growth and Death, Nervous and Sensory Systems, Signal Transduction
Alternate Names: C-C motif chemokine 5, small inducible cytokine A5, T cell specific protein RANTES, SIS delta, T cell specific protein P228, TCP228, Eosinophil-chemotactic cytokine, EoCP, RANTES(3-68), RANTES(4-68), CCL5, D17S136E, SCYA5Description:
RANTES is a chemoattractant for blood monocytes, memory T-helper cells and eosinophils. This cytokine causes the release of histamine from basophils and activates eosinophils. RANTES binds to CCR1, CCR3, CCR4 and CCR5, and is one of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form, RANTES (3-68), acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1 infection. The second processed form, RANTES (4-168), exhibits reduced chemotactic and HIV-suppressive activity compared to RANTES(1-68 and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. Recombinant Human Rantes produced in E. coli is a single non-glycosylated polypeptide chain containing 68 amino acids with a MW of 7,809.2 Da.
Source: E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Applications: E, WB
Application Notes:
rHuRantes is fully biologically active when compared to standard. The activity is determined by the chemoattract of human blood monocytes at a concentration between 1-8 ng/ml.
Accession No.: P13501
MW: 7809 Da
Sequence:
SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS
Purity:
Purity > 98% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Format:
Each vial contains 20 ug of lyophilized protein. Reconstitute with 0.2 ml sterile deionized water for a final concentration of 0.1 mg/ml.
Storage: Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
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