RANTES monoclonal, anti-human
€0.00
In stock
SKU
AC-ABG10473
Catalog Number: AC-ABG10473
Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
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Size(s): 500 µg
Host: Mouse
Applications: WB, IHC, E
Datasheet
Request Information
Source:
Produced in BALB/c mice using highly pure (>98%) human RANTES as the immunizing antigen. This IgG2b kappa antibody was purified from ascites fluid by Protein A affinity chromatography.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant human RANTES when used with BioGems biotinylated antigen affinity purified anti-human RANTES (60-285BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
*Additional Immunostaining data available. Please contact Tech Support for information.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 3.0-5.0 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Produced in BALB/c mice using highly pure (>98%) human RANTES as the immunizing antigen. This IgG2b kappa antibody was purified from ascites fluid by Protein A affinity chromatography.
Western Blot:
To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
Sandwich ELISA:
In a sandwich ELISA (assuming 100 μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant human RANTES when used with BioGems biotinylated antigen affinity purified anti-human RANTES (60-285BT) as the detection antibody at a concentration of approximately 0.5-1.0 μg/ml.
Immunohistochemistry:
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
*Additional Immunostaining data available. Please contact Tech Support for information.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/ml), a concentration of 3.0-5.0 μg/ml of this antibody is required.
Formulation: A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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