RAP1GAP polyclonal, anti-human, mouse
€305.00
In stock
SKU
BS5883
Background:
Rap1 GTPase activating protein (Rap1GAP) specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between Gα z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the α subunit, and also attenuates the ability of activated Gα z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to Gα z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
Alternative Name:
Rap1 GTPase-activating protein 1, Rap1GAP, Rap1GAP1, KIAA0474, RAP1GA1
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: RAP1GAP polyclonal antibody detects endogenous levels of RAP1GAP protein.
Immunogen:
Synthetic peptide, corresponding to the 525-570 of Human RAP1GAP.
MW: ~ 73, 89 kDa
Swis Prot.: P47736
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Rap1 GTPase activating protein (Rap1GAP) specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between Gα z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the α subunit, and also attenuates the ability of activated Gα z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to Gα z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
Alternative Name:
Rap1 GTPase-activating protein 1, Rap1GAP, Rap1GAP1, KIAA0474, RAP1GA1
Application Dilution: WB: 1:500~1:1000, IHC: 1:50~1:200
Specificity: RAP1GAP polyclonal antibody detects endogenous levels of RAP1GAP protein.
Immunogen:
Synthetic peptide, corresponding to the 525-570 of Human RAP1GAP.
MW: ~ 73, 89 kDa
Swis Prot.: P47736
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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