Rat 4-Hydroxynonenal (HNE)ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HNE protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HNE. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Metabolic pathway, Infection immunity, Hormone metabolism, Dermatology,

Alternative Names:
4-HNE, HNE, 4-hydroxy-2-nonenal, 5-Hydroxynonenal

Sample Type:
Serum, plasma, tissue homogenates

Assay Type: Competitive Inhibition

Standard: 1000 pg/mL

Assay Length: 2h

Assay Length: For Research Use Only