Rat a1AT (Alpha-1-Antitrypsin) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-Antitrypsin(a1AT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-Antitrypsin(a1AT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-Antitrypsin(a1AT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-Antitrypsin(a1AT) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Infection immunity, Hepatology

Alternative Names:
SERPINA1, SPAAT, A1-AT, PI, Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1, Alpha-1 protease inhibitor, Short peptide from AAT, Serpin A1

Sample Type:
Serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 500 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only