Rat Acly (ATP-citRate synthase) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acly(ATP-citRate synthase). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acly(ATP-citRate synthase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acly(ATP-citRate synthase), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acly(ATP-citRate synthase) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabonomics

Alternative Names:
ACL, ACLY, ATP citrate (pro S ) lyase, ATP citrate lyase, ATP citrate synthase, ATPCL, Citrate cleavage enzyme, CLATP

Sample Type:
tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 40 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only