Rat CFH (Complement Factor H) ELISA Kit

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Complement Factor H(CFH) protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor H(CFH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor H(CFH) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway, Infection immunity, Immune molecule,

Alternative Names:
CF-H, FH, FHL1, ARMD4, ARMS1, CFHL3, HF1, HF2, HUS, H Factor 2, Age-Related Maculopathy Susceptibility 1, Adrenomedullin binding protein

Sample Type:
Serum, plasma and other biological fluids.

Assay Type: Competitive Inhibition

Standard: 1000 ng/mL

Assay Length: 2h

Assay Length: For Research Use Only