Rat CYBb (Cytochrome b-245 Beta Polypeptide) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome b-245 Beta Polypeptide(CYBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome b-245 Beta Polypeptide(CYBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome b-245 Beta Polypeptide(CYBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome b-245 Beta Polypeptide(CYBb) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway

Alternative Names:
CGD, GP91PHOX, NOX2, Neutrophil cytochrome b91, Cytochrome b558 beta, Heme-binding membrane glycoprotein gp91phox, Superoxide-generating NADPH oxidase heavy chain

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only