Rat eIF4E (eukaryotic initiation factor 4E) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to eIF4E(eukaryotic initiation factor 4E). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to eIF4E(eukaryotic initiation factor 4E). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain eIF4E(eukaryotic initiation factor 4E), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of eIF4E(eukaryotic initiation factor 4E) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Breast Cancer

Alternative Names:
CBP, eIF 4E, eIF 4F 25 kDa subunit, EIF4E, EIF4E1, EIF4EL1, EIF4F

Sample Type:
serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 6000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only