Rat GP2 (Glycoprotein 2, Zymogen Granule Membrane) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein 2, Zymogen Granule Membrane(GP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycoprotein 2, Zymogen Granule Membrane(GP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein 2, Zymogen Granule Membrane(GP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycoprotein 2, Zymogen Granule Membrane(GP2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Endocrinology

Alternative Names:
ZAP75, Pancreatic Secretory Granule Membrane Major Glycoprotein GP2, Pancreatic zymogen granule membrane protein GP-2

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only