Rat GSK3a (Glycogen Synthase Kinase 3 Alpha) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen Synthase Kinase 3 Alpha(GSK3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycogen Synthase Kinase 3 Alpha(GSK3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycogen Synthase Kinase 3 Alpha(GSK3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glycogen Synthase Kinase 3 Alpha(GSK3a) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Enzyme & Kinase, Metabolic pathway, Endocrinology,

Alternative Names:
Serine/threonine-protein kinase GSK3A

Sample Type:
tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 40 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only