Rat GSTm1 (Glutathione S Transferase Mu 1) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione S Transferase Mu 1(GSTm1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutathione S Transferase Mu 1(GSTm1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione S Transferase Mu 1(GSTm1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutathione S Transferase Mu 1(GSTm1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
GST-M1, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MU1, GST HB subunit 4

Sample Type:
Serum, plasma and other biological fluids.

Assay Type: Sandwich

Standard: 400 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only