Rat HMGCS (Hydroxymethylglutaryl Coenzyme A Synthase) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydroxymethylglutaryl Coenzyme A Synthase(HMGCS). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hydroxymethylglutaryl Coenzyme A Synthase(HMGCS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydroxymethylglutaryl Coenzyme A Synthase(HMGCS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxymethylglutaryl Coenzyme A Synthase(HMGCS) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Metabolic pathway, Endocrinology

Alternative Names:
HMGCS1, 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 1,Soluble, HMG-CoA Synthase

Sample Type:
Tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only