Rat HNF1b (Hepatocyte Nuclear Factor 1 Beta) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Nuclear Factor 1 Beta(HNF1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatocyte Nuclear Factor 1 Beta(HNF1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatocyte Nuclear Factor 1 Beta(HNF1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hepatocyte Nuclear Factor 1 Beta(HNF1b) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine

Alternative Names:
FJHN, TCF2, HNF1βeta, HNF2, LFB3, LF-B3, MODY5, VHNF1, Transcription Factor 2,Hepatic, Variant Hepatic Nuclear Factor, Homeoprotein LFB3

Sample Type:
Tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only