Rat IL-18 ELISA Kit
€0.00
In stock
SKU
BEK1126
Catalog Number: BEK1126
Size(s): 96 tests/kit
Standard Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 1 pg/ml
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Size(s): 96 tests/kit
Standard Range: 15.6 pg/ml - 1000 pg/ml
Sensitivity: < 1 pg/ml
Request Information
Introduction:
Interleukin-18 (IL18), also known as interferon-gamma inducing factor, in human s is encoded by the IL18 gene, this gene contains 6 exons, and maps to 11q22.2-q22.3. It is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. It works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 has been implicated as an inflammatory mediator of Hashimoto's thyroiditis, the most common cause of autoimmune hypothyroidism. IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-18 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-18 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-18 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-18 can be calculated.
Application:
For quantitative detection of IL-18 in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Interleukin-18 (IL18), also known as interferon-gamma inducing factor, in human s is encoded by the IL18 gene, this gene contains 6 exons, and maps to 11q22.2-q22.3. It is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. It works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 has been implicated as an inflammatory mediator of Hashimoto's thyroiditis, the most common cause of autoimmune hypothyroidism. IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders.
Principle of the Assay:
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-18 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-18 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-18 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-18 can be calculated.
Application:
For quantitative detection of IL-18 in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
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