Rat IL15Ra (Interleukin 15 Receptor Alpha) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL15Ra(Interleukin 15 Receptor Alpha). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL15Ra(Interleukin 15 Receptor Alpha). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL15Ra(Interleukin 15 Receptor Alpha), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL15Ra(Interleukin 15 Receptor Alpha) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Immunology

Alternative Names:
CD215, IL15-RA, Interleukin 15 Receptor Alpha

Sample Type:
serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 2000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only