Rat JAG1 (Jagged 1 Protein) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Jagged 1 Protein(JAG1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Jagged 1 Protein(JAG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Jagged 1 Protein(JAG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Jagged 1 Protein(JAG1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, CD & Adhesion molecule, Tumor immunity, Hematology,

Alternative Names:
CD339, AGS, AHD, AWS, HJ1, JAGL1, Alagille Syndrome

Sample Type:
Tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 3.5h

Assay Length: For Research Use Only