Rat L1CAM (L1-Cell Adhesion Molecule) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L1-Cell Adhesion Molecule(L1CAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to L1-Cell Adhesion Molecule(L1CAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain L1-Cell Adhesion Molecule(L1CAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of L1-Cell Adhesion Molecule(L1CAM) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: CD & Adhesion molecule, Tumor immunity, Infection immunity, Neuro science

Alternative Names:
CD171, L1-CAM, CAML1, HSAS, HSAS1, MASA, MIC5, N-CAML1, S10, SPG1, Antigen Identified By Monoclonal Antibody R1

Sample Type:
Serum, plasma and other biological fluids.

Assay Type: Sandwich

Standard: 5000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only