Rat LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Lipoprotein Associated(LpPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Lipoprotein Associated(LpPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Lipoprotein Associated(LpPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Lipoprotein Associated(LpPLA2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Metabolic pathway, Infection immunity

Alternative Names:
PLA2G7, PAF-AH, PAFAH, Lp-PLA2, LDL-PLA2, Platelet Activating Factor Acetylhydrolase,Plasma, Phospholipase A2,Group VII, LDL-associated phospholipase A2

Sample Type:
Serum, plasma, tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 800 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only