Rat MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Metabolic pathway

Alternative Names:
LC3B, MAP1LC3B, MAP1ALC3, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only