Rat MGMT (O-6-Methylguanine DNA Methyltransferase) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-6-Methylguanine DNA Methyltransferase(MGMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-6-Methylguanine DNA Methyltransferase(MGMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-6-Methylguanine DNA Methyltransferase(MGMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of O-6-Methylguanine DNA Methyltransferase(MGMT) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, Enzyme & Kinase, Tumor immunity,

Alternative Names:
Methylated-DNA--Protein-Cysteine Methyltransferase, 6-O-methylguanine-DNA methyltransferase, Methylated-DNA--protein-cysteine methyltransferase

Sample Type:
Tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 5000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only