Rat MIP1g (Macrophage Inflammatory Protein 1 Gamma) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Gamma(MIP1g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 1 Gamma(MIP1g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 1 Gamma(MIP1g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 1 Gamma(MIP1g) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Cytokine, Infection immunity, Human gene deletion

Alternative Names:
CCL9, CCL10, SCYB9, CCF18, MRP2, C-C motif chemokine 9, Macrophage inflammatory protein-related protein 2, Small-inducible cytokine A9

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 1000 pg/mL

Assay Length: 4.5h

Assay Length: For Research Use Only