Rat Normal Adipose Lysate
€145.00
In stock
SKU
450149
Description:
Rat adipose tissue from outbred Sprague Dawley Albino rat. Adipose tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Alternate Names: total protein adipose - rat
Format: Lysate is supplied at 2 mg/ml in 1X SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Applications: WB
Application Notes: WB: Boil the mixture for 10 min before loading. Load 5-20 ug lysate per lane.
Storages: Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -80°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment.
Rat adipose tissue from outbred Sprague Dawley Albino rat. Adipose tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Alternate Names: total protein adipose - rat
Format: Lysate is supplied at 2 mg/ml in 1X SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% beta-mercaptoethanol.
Applications: WB
Application Notes: WB: Boil the mixture for 10 min before loading. Load 5-20 ug lysate per lane.
Storages: Store at 2-8°C for continuous use. For extended storage, freeze working aliquots at -80°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment.
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