Rat NT5C2 (5'-Nucleotidase, Cytosolic II) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-Nucleotidase, Cytosolic II(NT5C2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5'-Nucleotidase, Cytosolic II(NT5C2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5'-Nucleotidase, Cytosolic II(NT5C2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5'-Nucleotidase, Cytosolic II(NT5C2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
NT5B, PNT5, GMP, cN-II, NT5CP, Purine 5' Nucleotidase, 5'-Nucleotidase(Purine),Cytosolic Type B, Cytosolic purine 5'-nucleotidase

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only