Rat P4Ha1 (Prolyl-4-Hydroxylase Alpha Polypeptide I) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I(P4Ha1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I(P4Ha1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide I(P4Ha1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide I(P4Ha1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase

Alternative Names:
Procollagen-Proline,2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase),Alpha Polypeptide I

Sample Type:
Tissue homogenates and other biological fluids.

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only