Rat PROCR (Protein C Receptor, Endothelial) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein C Receptor, Endothelial(PROCR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein C Receptor, Endothelial(PROCR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein C Receptor, Endothelial(PROCR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein C Receptor, Endothelial(PROCR) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, CD & Adhesion molecule, Hematology,

Alternative Names:
CD201, CCCA , CCD41, BA42O4.2, EPCR, Endothelial Protein C Receptor, Cell Cycle, Centrosome-Associated Protein, Activated Protein C Receptor

Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Type: Sandwich

Standard: 100 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only