Rat PTGES (Prostaglandin E Synthase, Microsomal) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin E Synthase, Microsomal(PTGES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin E Synthase, Microsomal(PTGES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin E Synthase, Microsomal(PTGES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin E Synthase, Microsomal(PTGES) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Enzyme & Kinase, Metabolic pathway, Infection immunity

Alternative Names:
MGST1L1, MGST-IV, MGST1-L1, PGES, PIG12, PP102, PP1294, TP53I12, PGE Synthase, Tumor Protein p53 Inducible Protein 12, Glutathione S-Transferase 1-Like 1, MGST1-Like 1

Sample Type:
Tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only