Rat RAGE ELISA Kit
€0.00
In stock
SKU
BEK1373
Catalog Number: BEK1373
Size(s): 96 tests/kit
Standard Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: <5 pg/ml
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Size(s): 96 tests/kit
Standard Range: 31.2 pg/ml - 2000 pg/ml
Sensitivity: <5 pg/ml
Request Information
Introduction:
RAGE (receptor for advanced glycation end-products), also called AGER, is a 35 kilodalton transmembrane receptorof the immunoglobulin super family which was first characterized in 1992 by Neeper et al.Its name comes from its ability to bind advanced glycation end-products (AGEs), which include chiefly glycoproteins, the glycans of which have been modified non-enzymatically through the Maillard reaction. In view of its inflammatory function in innate immunity and its ability to detect a class of ligands through a common structural motif, RAGE is often referred to as a pattern recognition receptor. RAGE also has at least one other agonistic ligand: high mobility group protein B1 (HMGB1).
Principle of the Assay:
This kit was based onsandwich enzyme-linked immune-sorbent assay technology. Anti-RAGE polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RAGE polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RAGE amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of RAGE can be calculated.
Application:
For quantitative detection of RAGE in Rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
RAGE (receptor for advanced glycation end-products), also called AGER, is a 35 kilodalton transmembrane receptorof the immunoglobulin super family which was first characterized in 1992 by Neeper et al.Its name comes from its ability to bind advanced glycation end-products (AGEs), which include chiefly glycoproteins, the glycans of which have been modified non-enzymatically through the Maillard reaction. In view of its inflammatory function in innate immunity and its ability to detect a class of ligands through a common structural motif, RAGE is often referred to as a pattern recognition receptor. RAGE also has at least one other agonistic ligand: high mobility group protein B1 (HMGB1).
Principle of the Assay:
This kit was based onsandwich enzyme-linked immune-sorbent assay technology. Anti-RAGE polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RAGE polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RAGE amount of sample captured in plate. Read the O. D. absorbance at 450nm in a microplate reader, and then the concentration of RAGE can be calculated.
Application:
For quantitative detection of RAGE in Rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.
Storage and Expiration:
Store at 2-8℃ for 6months, or at -20℃for 12 months.
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