Rat TAOK2 (Serine/threonine-protein kinase TAO2) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAOK2(Serine/threonine-protein kinase TAO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TAOK2(Serine/threonine-protein kinase TAO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TAOK2(Serine/threonine-protein kinase TAO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TAOK2(Serine/threonine-protein kinase TAO2) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Functional Studies

Alternative Names:
hKFC C, KIAA0881, Kinase from chicken homolog C, MAP3K17, PSK, PSK 1, PSK1, TAO kinase 2, TAO1, TAO2, TAOK2

Sample Type:
serum, plasma and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only