Rat TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit
Catalog Number: ACE-E3832
Size: 96 tests/kit
Detection Range: 12.50-800 pg/mL
Sensitivity: 7.50 pg/mL
Datasheet
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Size: 96 tests/kit
Detection Range: 12.50-800 pg/mL
Sensitivity: 7.50 pg/mL
Datasheet
Request Information
Price on request
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You are enquiring about Rat TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit
Description:
This CLIA kit applies to the in vitro quantitative determination of Rat TGF-β1 concentrations in serum, plasma and other biological fluids.
Assay Principles: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-β1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TGF-β1. You can calculate the concentration of Rat TGF-β1 in the samples by comparing the RLU value of the samples to the standard curve.
Species reactivity:
Rat
Sample Type: serum, plasma, cell lysates, tissue homogenates, cell culture supernatant or other biological fluids
Storage instruction: -20˚C and 4˚C for 6 months
This CLIA kit applies to the in vitro quantitative determination of Rat TGF-β1 concentrations in serum, plasma and other biological fluids.
Assay Principles: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-β1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TGF-β1. You can calculate the concentration of Rat TGF-β1 in the samples by comparing the RLU value of the samples to the standard curve.
Species reactivity:
Rat
Sample Type: serum, plasma, cell lysates, tissue homogenates, cell culture supernatant or other biological fluids
Storage instruction: -20˚C and 4˚C for 6 months
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