Rat TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 8(TNFRSF8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Receptor Superfamily, Member 8(TNFRSF8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Receptor Superfamily, Member 8(TNFRSF8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Receptor Superfamily, Member 8(TNFRSF8) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Signal transduction, CD & Adhesion molecule, Apoptosis, Tumor immunity, Reproductive science,

Alternative Names:
CD30, KI-1, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30

Sample Type:
serum, plasma, tissue homogenates and other biological fluids

Assay Type: Sandwich

Standard: 20 ng/mL

Assay Length: 4.5h

Assay Length: For Research Use Only