Rat XIAP (Inhibitor Of Apoptosis Protein, X-Linked) ELISA Kit

Test Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inhibitor Of Apoptosis Protein, X-Linked(XIAP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Inhibitor Of Apoptosis Protein, X-Linked(XIAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inhibitor Of Apoptosis Protein, X-Linked(XIAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inhibitor Of Apoptosis Protein, X-Linked(XIAP) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area: Apoptosis

Alternative Names:
BIRC4, API3, ILP1, MIHA, XLP2, Baculoviral IAP Repeat-Containing 4, E3 ubiquitin-protein ligase XIAP, IAP-like, Inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein

Sample Type:
tissue homogenates, cell lysates and other biological fluids

Assay Type: Sandwich

Standard: 10 ng/mL

Assay Length: 3.5h

Assay Length: For Research Use Only