RBP (clone G4E4), anti-human, mouse, rat, chimpanzee, goat, monkey, rabbit, rhesus

Source:
A BALB/c mouse was immunized with human retinol binding protein purified from plasma.
Fusion partner: SP2/0.

Specifications:
G4E4 recognizes an epitope within the 74-182 C-terminal sequence (11kD peptide fragment) of human serum Cellular Retinol Binding Protein 1 (CRBP 1), a single-chain glycoprotein belonging to the superfamily of hydrophobic molecule transporter proteins, which is responsible for transport of retinol (vitamin A1) from the liver to peripheral target tissues, like the eye, where it mediates the cellular uptake. CRBP 1 is synthesized by hepatic parenchymal cells where it becomes bound to its ligand retinol and is then released into the circulation, where it binds further to the protein transthyretin, to form a transporting complex, which is big enough not to be lost by filtration through the kidney glomeruli. It is detected in nearly all tissues with higher expression in adult ovary, pancreas, pituitary gland, adrenal gland, and fetal liver.

Species reactivity:
Positive: chimpanzee, goat, human, monkey, mouse, rabbit, rat, rhesus.

Applications:
Serology by ELISA to measure the availability of RBP directly and retinol indirectly. Sera from patients with breast and ovarian carcinomas contain a significantly lower amount of RBP than sera from normal individuals (unpublished data).
ELISA: +
Frozen sections: +
Paraffin sections: Citrate
Western blot: +

Positive control: Liver.

References:
- Reddy B. et al. Biochem. Int.21: 367-376 (1990).
- Reddy B. et al. Molec. Immunol.29: 511-516 (1992).
- Reddy B. et al. Molec. Immunol.30: 1355-1360 (1993)