RBX1 polyclonal, anti-human, mouse

RBX1 polyclonal, anti-human, mouse

€388.00
In stock
SKU
BS7297
Catalog Number: BS7297
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IF

Datasheet
Background:
RING-box protein 1 (RBX1 or ROC1) is an essential component of two distinct but structurally related E3 ubiquitin ligase complexes, the SCF complex and the CBC (VHL) complex (1). RBX1 mediates the neddylation of CUL1, which activates SCF E3 ligase by facilitating the ubiquitin transfer from E2 to substrates (2-4). The RING finger domain of RBX1 is required for ubiquitin ligation (5). Two evolutionarily conserved mammalian RBX family members, RBX1/ROC1 and RBX2/ROC2/SAG, have been identified (5). RBX1 is constitutively expressed and binds to CUL2/VHL, while stress-inducible RBX2 binds to CUL5/SOCS (6).

Alternative Name:
E3 ubiquitin-protein ligase RBX1, FLJ60363, MGC13357, MGC1481, OTTHUMP00000028983, Protein ZYP, Rbx 1, Rbx1, RBX1_HUMAN, Regulator of Cullins 1, Ring box 1, Ring box 1 E3 ubiquitin protein ligase, RING box protein 1, RING finger protein 75, RING finger protein, RING-box protein 1, Ringbox protein 1, RNF 75, RNF75, ROC 1, ZYP protein,

Application Dilution: WB: 1:500~1:2000, IF: 1:50~1:200

Specificity: RBX1 polyclonal antibody detects endogenous levels of RBX1 protein.

Immunogen:
Recombinant full length Human RBX1.

MW: ~12 kDa

Swis Prot.: P62877

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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