Rde-4 Antibody (N-term) Blocking Peptide
€363.00
In stock
SKU
AC-BP1969a
Background:
Double-stranded (ds) RNA is a potent sequence-specific inhibitor of gene function. RNA interference (RNAi) is a cellular defense mechanism that uses double-stranded RNA (dsRNA) as a sequence-specific trigger to guide the degradation of homologous single-stranded RNAs. RNAi is a multistep process involving several proteins and at least one type of RNA intermediate, a population of small 21??5 nt RNAs (called siRNAs) that are initially derived from cleavage of the dsRNA trigger. Genetic studies have implicated several RNA interference-deficient (rde) family members in germline maintenance and development, and several simple loss of function mutants have been identified. Family members rde-1 and rde-4 are required for RNAi but are not essential for organismal viability. While rde-1 and rde-4 are distinct from other RNAi-deficient family members both both for their inability to mobilize transposons and lack of chromosome loss, each appears to have a distinct role in the interference mechanism. Evidence indicates that rde-4 is involved before or during production of siRNAs, whereas rde-1 acts after the siRNAs have been formed.
Target/Specificity:
The synthetic peptide sequence used to generate the antibody AP1969a was selected from the N-term region of human Rde-4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Gene Name: G5EBF5
Gene ID: 176438
Primary Accession: G5EBF5
Other Accession: Q22617
Format: Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.
Double-stranded (ds) RNA is a potent sequence-specific inhibitor of gene function. RNA interference (RNAi) is a cellular defense mechanism that uses double-stranded RNA (dsRNA) as a sequence-specific trigger to guide the degradation of homologous single-stranded RNAs. RNAi is a multistep process involving several proteins and at least one type of RNA intermediate, a population of small 21??5 nt RNAs (called siRNAs) that are initially derived from cleavage of the dsRNA trigger. Genetic studies have implicated several RNA interference-deficient (rde) family members in germline maintenance and development, and several simple loss of function mutants have been identified. Family members rde-1 and rde-4 are required for RNAi but are not essential for organismal viability. While rde-1 and rde-4 are distinct from other RNAi-deficient family members both both for their inability to mobilize transposons and lack of chromosome loss, each appears to have a distinct role in the interference mechanism. Evidence indicates that rde-4 is involved before or during production of siRNAs, whereas rde-1 acts after the siRNAs have been formed.
Target/Specificity:
The synthetic peptide sequence used to generate the antibody AP1969a was selected from the N-term region of human Rde-4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Gene Name: G5EBF5
Gene ID: 176438
Primary Accession: G5EBF5
Other Accession: Q22617
Format: Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed.
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