Reactive Oxygen Species (ROS) Fluorometric Assay Kit

Detection Principle:
DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Instrument: Fluorescence Microplate reader, Fluorescence Microscope, Flow Cytometry
Assay Time: 60 min

Tested Sample Types: Fresh tissue,cultured cells

Research Area's: Oxidative stress

Shelf Life: 6 Months

Storage:
Reagent1:-20℃;Others:2-8℃

Citations:


Detection Principle: https://www.mdpi.com/1422-0067/20/15/3649
https://assets.researchsquare.com/files/b5a35004-8e2f-40e8-9092-22286ec9bb25/v1/Manuscript.pdf
http://www.plantarchives.com/file/SPL%20ISSUE%20SUPP%202,2019/123%20(695-701).pdf
https://pubmed.ncbi.nlm.nih.gov/32474680/
https://www.tandfonline.com/doi/full/10.1080/13880209.2021.1889002
https://doi.org/10.1016/j.sjbs.2021.02.007
https://doi.org/10.1016/j.jsps.2020.12.003
https://www.researchgate.net/publication/339310434
https://pubmed.ncbi.nlm.nih.gov/31349735/
https://doi.org/10.3390/molecules26185546