Recombinant e.coli GrpE protein (V44I)

Recombinant e.coli GrpE protein (V44I)

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In stock
SKU
GRP0701
Catalog NumberGRP0701
Applications:
SDS-PAGE
Datasheet
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Description
GrpE, co-chaperone of E. coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, Dna K and GrpE are required for fully efficient folding. Recombinant GrpE protein was overexpressed in E. coli and purified by using the conventional column chromatography techniques.Alternative Names
HSP-70 cofactor, Heat shock protein B25.3, HSP24, GrpE, HSP 70 cofactor.Concentration
1mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaClStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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