Recombinant E.coli rnpA protein
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In stock
SKU
ATGP4054
Description
rnpA, also known as Rnase P protein, is an essential enzyme consisting of the C5 protein (encoded by rnpA) and the catalytic M1 RNA (encoded by rnpB) subunits. rnpA is ribonucleoprotein that catalyzes the removal of the 50-leader elements of precursor tRNAs and generates the mature 50-end of tRNAs. This step is essential for the formation of functional tRNA molecules in bacteria, archaea and eukarya. More importantly, it has recently been demonstrated that RNase P is required for the endonucleolytic separation of certain polycistronic tRNA transcripts such as valV valW, leuQ leuP leuV and secG leuU. Thus, it was hypothesized that the essential function of RNase P might be related to the complete absence of a particular tRNAthat was dependent on the enzyme for initial separation from polycistronic transcripts. Recombinant E.coli rnpA, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
ECK3696, Rnase P protein, RnaseP protein, b3704, JW3681, Ribonuclease P protein component, EC 3.1.26.5, Protein C5Concentration
0.25mg/ml (determined by absorbance at 280nm)Concentration
Liquid. Phosphate-Buffered Saline (pH 7.4) containing 10% GlycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
rnpA, also known as Rnase P protein, is an essential enzyme consisting of the C5 protein (encoded by rnpA) and the catalytic M1 RNA (encoded by rnpB) subunits. rnpA is ribonucleoprotein that catalyzes the removal of the 50-leader elements of precursor tRNAs and generates the mature 50-end of tRNAs. This step is essential for the formation of functional tRNA molecules in bacteria, archaea and eukarya. More importantly, it has recently been demonstrated that RNase P is required for the endonucleolytic separation of certain polycistronic tRNA transcripts such as valV valW, leuQ leuP leuV and secG leuU. Thus, it was hypothesized that the essential function of RNase P might be related to the complete absence of a particular tRNAthat was dependent on the enzyme for initial separation from polycistronic transcripts. Recombinant E.coli rnpA, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
ECK3696, Rnase P protein, RnaseP protein, b3704, JW3681, Ribonuclease P protein component, EC 3.1.26.5, Protein C5Concentration
0.25mg/ml (determined by absorbance at 280nm)Concentration
Liquid. Phosphate-Buffered Saline (pH 7.4) containing 10% GlycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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