Recombinant human ATG4B protein
€0.00
In stock
SKU
ATGP1431
Description
ATG4B, also known as cysteine protease ATG4B, is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE) -conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Cysteine protease ATG4B, AuTL1, Autophagin-1Concentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSFStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
ATG4B, also known as cysteine protease ATG4B, is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE) -conjugated form, and has the capacity for the binding to autophagosomes. Recombinant human ATG4B protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Cysteine protease ATG4B, AuTL1, Autophagin-1Concentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSFStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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