Recombinant Human BMP-15

Synonyms: BMP 15, BMP15, BMP-15, bone morphogenetic protein 15, GDF 9B, GDF9B, ODG2, POF4

Mol. Weight: 35 kda

Activity: Measured by its ability to induce Smad3 phosphorylation in P19 mouse embryonal carcinoma cells. Approximately 25 ng/mL. can effectively induce Smad3 phosphorylation.

Uniprot ID: O95972

Formulation: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4.

Purity: Greater than 90% as determined by SDS-PAGE.

Reconstitution: Centrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg/ml by gently pipetting 2-3 times, don't vortex.

Storage: The lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week under sterile condition.For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months. Avoid repeated freeze/thaw cycle.