Recombinant human DPH2 protein

Recombinant human DPH2 protein

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In stock
SKU
ATGP3582
Catalog NumberATGP3582
Applications:
SDS-PAGE
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Description
DPH2, also known as diphthamide biosynthesis protein 2, is a homodimer and each of its monomers can bind a [4Fe-4S] cluster. It is the target of ADP ribosylating diphtheria toxin (DT) and Pseudomonas exotoxin A (PE). It was identified by its ability to complement a diphthamide mutant strain, and thus functions in diphthamide biosynthesis. Its loss pre-activates NF-kB and death receptor pathways and renders MCF7 cells hypersensitive to tumor necrosis factor. Recombinant human DPH2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.Alternative Names
Diphthamide biosynthesis protein 2, DPH2, DPH2L2Concentration
0.5mg/ml (determined by absorbance at 280nm)Concentration
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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