Recombinant human DR1 protein
€0.00
In stock
SKU
ATGP0669
Description
DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I. Recombinant human DR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatographyAlternative Names
Down-regulator of transcription 1 TBP-binding, NC2, NC2-BETA, Down-regulator of transcription 1, TBP-bindingConcentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 0.1mM PMSF ,10% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I. Recombinant human DR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatographyAlternative Names
Down-regulator of transcription 1 TBP-binding, NC2, NC2-BETA, Down-regulator of transcription 1, TBP-bindingConcentration
0.5mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 100mM NaCl, 0.1mM PMSF ,10% glycerolStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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