Recombinant human EGLN3/PHD3 protein
€0.00
In stock
SKU
ATGP1609
Description
EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the alpha subunit of hypoxia-inducible factor-alpha, which targets hypoxia-inducible factor-alpha for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Egl nine homolog 3, HIFPH3, PHD3Concentration
0.25mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5mM DTT, 2mM EDTAStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the alpha subunit of hypoxia-inducible factor-alpha, which targets hypoxia-inducible factor-alpha for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.Alternative Names
Egl nine homolog 3, HIFPH3, PHD3Concentration
0.25mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5mM DTT, 2mM EDTAStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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