Recombinant human GATC protein
€0.00
In stock
SKU
ATGP2183
Description
GATC allows the formation of correctly charged Gln-tRNA (Gln) through the transamidation of misacylated Glu-tRNA (Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA (Gln). This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
Glutamyl-tRNA(Gln) amidotransferase subunit C homolog, Glutamyl-tRNA(Gln) amidotransferase, subunit C homolog, 15E1.2Concentration
0.25mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
GATC allows the formation of correctly charged Gln-tRNA (Gln) through the transamidation of misacylated Glu-tRNA (Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA (Gln). This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.Alternative Names
Glutamyl-tRNA(Gln) amidotransferase subunit C homolog, Glutamyl-tRNA(Gln) amidotransferase, subunit C homolog, 15E1.2Concentration
0.25mg/ml (determined by Bradford assay)Concentration
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTTStorage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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